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pcmv6 entry plasmid  (OriGene)


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    Structured Review

    OriGene pcmv6 entry plasmid
    Pcmv6 Entry Plasmid, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1670 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv6+entry+vector/pCMV6-Entry+Mammalian+Expression+Vector/pmc13217847-87-0-3
    Average 96 stars, based on 1670 article reviews
    pcmv6 entry plasmid - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Isoform-specific aldehyde dehydrogenase inhibitors
    Article Snippet: .. Immunofluorescence Microscopy ALDH1A3−/− A375 cells were seeded into a 6-well plate at a density of 6×105 cells/well and cultured for 24 h. The cells were then transfected with either pCMV6-entry vector (Origene, PS100001) or pCMV6-ALDH1B1 (created by obtaining the ALDH1B1 sequence with the mitochondria targeting signal from SW480 cDNA and subcloning it into the PCMV6-entry vector by Gibson assembly) using Fugene HD (Promega) at a 3:1 DNA:Fugene HD ratio according to the manufacturer's protocols. ..

    Microscopy:

    Article Title: Isoform-specific aldehyde dehydrogenase inhibitors
    Article Snippet: .. Immunofluorescence Microscopy ALDH1A3−/− A375 cells were seeded into a 6-well plate at a density of 6×105 cells/well and cultured for 24 h. The cells were then transfected with either pCMV6-entry vector (Origene, PS100001) or pCMV6-ALDH1B1 (created by obtaining the ALDH1B1 sequence with the mitochondria targeting signal from SW480 cDNA and subcloning it into the PCMV6-entry vector by Gibson assembly) using Fugene HD (Promega) at a 3:1 DNA:Fugene HD ratio according to the manufacturer's protocols. ..

    Cell Culture:

    Article Title: Isoform-specific aldehyde dehydrogenase inhibitors
    Article Snippet: .. Immunofluorescence Microscopy ALDH1A3−/− A375 cells were seeded into a 6-well plate at a density of 6×105 cells/well and cultured for 24 h. The cells were then transfected with either pCMV6-entry vector (Origene, PS100001) or pCMV6-ALDH1B1 (created by obtaining the ALDH1B1 sequence with the mitochondria targeting signal from SW480 cDNA and subcloning it into the PCMV6-entry vector by Gibson assembly) using Fugene HD (Promega) at a 3:1 DNA:Fugene HD ratio according to the manufacturer's protocols. ..

    Transfection:

    Article Title: Isoform-specific aldehyde dehydrogenase inhibitors
    Article Snippet: .. Immunofluorescence Microscopy ALDH1A3−/− A375 cells were seeded into a 6-well plate at a density of 6×105 cells/well and cultured for 24 h. The cells were then transfected with either pCMV6-entry vector (Origene, PS100001) or pCMV6-ALDH1B1 (created by obtaining the ALDH1B1 sequence with the mitochondria targeting signal from SW480 cDNA and subcloning it into the PCMV6-entry vector by Gibson assembly) using Fugene HD (Promega) at a 3:1 DNA:Fugene HD ratio according to the manufacturer's protocols. ..

    Article Title: Tumor-intrinsic KLRC1 exerts tumor-suppressive functions in colorectal cancer.
    Article Snippet: .. 2.6 Plasmid and siRNA transfection To generate a KLRC1 overexpression construct, the open reading frame (ORF) sequence was cloned into the pCMV6-entry vector (OriGene Technologies). .. For KLRC1 knockdown, transfection involved administering 7.2 μL of targeted siRNA (20 μM) with Lipofectamine 3000 reagent (Thermo Fisher Scientific) following the manufacturer's protocol.

    Plasmid Preparation:

    Article Title: Isoform-specific aldehyde dehydrogenase inhibitors
    Article Snippet: .. Immunofluorescence Microscopy ALDH1A3−/− A375 cells were seeded into a 6-well plate at a density of 6×105 cells/well and cultured for 24 h. The cells were then transfected with either pCMV6-entry vector (Origene, PS100001) or pCMV6-ALDH1B1 (created by obtaining the ALDH1B1 sequence with the mitochondria targeting signal from SW480 cDNA and subcloning it into the PCMV6-entry vector by Gibson assembly) using Fugene HD (Promega) at a 3:1 DNA:Fugene HD ratio according to the manufacturer's protocols. ..

    Article Title: Functional analysis of O-GlcNAcylation by networking of OGT interactors and substrates.
    Article Snippet: All primers were purchased from Integrated DNA Technologies and all molecular biology supplies were purchased from New England Biolabs, unless otherwise indicated. .. The OGT lentiviral vector was produced by first cloning OGT with C-terminal FLAG and HA tags into the pCMV6 entry vector (Origene) using the NEBuilder HiFi DNA assembly and Q5 site-directed mutagenesis kits according to the manufacturer’s protocols. ..

    Article Title: A new combination of 5’- and 3’-untranslated regions increases the expression of mRNAs in vitro and in vivo
    Article Snippet: .. Supplementary Materials are available in the online version of the paper: https://vavilovj-icg.ru/download/pict-2025-29/appx24.pdf Fragments containing MCS and UTRs were synthesized by the Laboratory of Synthetic Biology at the Institute of Chemical Biology and Fundamental Medicine (ICBFM) SB RAS and cloned into the pCMV6-Entry vector (OriGene, USA; see Supplementary Fig. S1) by means of restriction sites Psp124BI and XmaI (SibEnzyme, Russia). ..

    Article Title: Lung cancer methylation markers and uses thereof
    Article Snippet: The expression construct for a gene disclosed herein is purchased from Origene in a form of TrueORF® cDNA clones in pCMV6-Entry vector. cDNAs are shuttled into pLenti-C-mGFP (Origene) to create a lentivector encoding a fusion protein between the desired gene and mGFP. .. The expression construct for a gene disclosed herein is purchased from Origene in a form of TrueORF® cDNA clones in pCMV6-Entry vector. cDNAs are shuttled into pLenti-C-mGFP (Origene) to create a lentivector encoding a fusion protein between the desired gene and mGFP. ..

    Article Title: GPR39-mediated molecular signaling by bile acids
    Article Snippet: .. Mouse GPR39 cDNA was cloned into the pCMV6-Entry vector (OriGene), with an in-frame fusion to mCherry via a P2A linker. ..

    Article Title: Tumor-intrinsic KLRC1 exerts tumor-suppressive functions in colorectal cancer.
    Article Snippet: .. 2.6 Plasmid and siRNA transfection To generate a KLRC1 overexpression construct, the open reading frame (ORF) sequence was cloned into the pCMV6-entry vector (OriGene Technologies). .. For KLRC1 knockdown, transfection involved administering 7.2 μL of targeted siRNA (20 μM) with Lipofectamine 3000 reagent (Thermo Fisher Scientific) following the manufacturer's protocol.

    Article Title: GPR39 mediated molecular signaling by bile acids.
    Article Snippet: .. Mouse GPR39 cDNA was cloned into the pCMV6-Entry vector (OriGene), with an in-frame fusion to mCherry via a P2A linker. ..

    Sequencing:

    Article Title: Isoform-specific aldehyde dehydrogenase inhibitors
    Article Snippet: .. Immunofluorescence Microscopy ALDH1A3−/− A375 cells were seeded into a 6-well plate at a density of 6×105 cells/well and cultured for 24 h. The cells were then transfected with either pCMV6-entry vector (Origene, PS100001) or pCMV6-ALDH1B1 (created by obtaining the ALDH1B1 sequence with the mitochondria targeting signal from SW480 cDNA and subcloning it into the PCMV6-entry vector by Gibson assembly) using Fugene HD (Promega) at a 3:1 DNA:Fugene HD ratio according to the manufacturer's protocols. ..

    Article Title: Tumor-intrinsic KLRC1 exerts tumor-suppressive functions in colorectal cancer.
    Article Snippet: .. 2.6 Plasmid and siRNA transfection To generate a KLRC1 overexpression construct, the open reading frame (ORF) sequence was cloned into the pCMV6-entry vector (OriGene Technologies). .. For KLRC1 knockdown, transfection involved administering 7.2 μL of targeted siRNA (20 μM) with Lipofectamine 3000 reagent (Thermo Fisher Scientific) following the manufacturer's protocol.

    Subcloning:

    Article Title: Isoform-specific aldehyde dehydrogenase inhibitors
    Article Snippet: .. Immunofluorescence Microscopy ALDH1A3−/− A375 cells were seeded into a 6-well plate at a density of 6×105 cells/well and cultured for 24 h. The cells were then transfected with either pCMV6-entry vector (Origene, PS100001) or pCMV6-ALDH1B1 (created by obtaining the ALDH1B1 sequence with the mitochondria targeting signal from SW480 cDNA and subcloning it into the PCMV6-entry vector by Gibson assembly) using Fugene HD (Promega) at a 3:1 DNA:Fugene HD ratio according to the manufacturer's protocols. ..

    Produced:

    Article Title: Functional analysis of O-GlcNAcylation by networking of OGT interactors and substrates.
    Article Snippet: All primers were purchased from Integrated DNA Technologies and all molecular biology supplies were purchased from New England Biolabs, unless otherwise indicated. .. The OGT lentiviral vector was produced by first cloning OGT with C-terminal FLAG and HA tags into the pCMV6 entry vector (Origene) using the NEBuilder HiFi DNA assembly and Q5 site-directed mutagenesis kits according to the manufacturer’s protocols. ..

    Cloning:

    Article Title: Functional analysis of O-GlcNAcylation by networking of OGT interactors and substrates.
    Article Snippet: All primers were purchased from Integrated DNA Technologies and all molecular biology supplies were purchased from New England Biolabs, unless otherwise indicated. .. The OGT lentiviral vector was produced by first cloning OGT with C-terminal FLAG and HA tags into the pCMV6 entry vector (Origene) using the NEBuilder HiFi DNA assembly and Q5 site-directed mutagenesis kits according to the manufacturer’s protocols. ..

    Mutagenesis:

    Article Title: Functional analysis of O-GlcNAcylation by networking of OGT interactors and substrates.
    Article Snippet: All primers were purchased from Integrated DNA Technologies and all molecular biology supplies were purchased from New England Biolabs, unless otherwise indicated. .. The OGT lentiviral vector was produced by first cloning OGT with C-terminal FLAG and HA tags into the pCMV6 entry vector (Origene) using the NEBuilder HiFi DNA assembly and Q5 site-directed mutagenesis kits according to the manufacturer’s protocols. ..

    Expressing:

    Article Title: Liver cancer methylation markers and uses thereof
    Article Snippet: Cell Culture and Gene Transfections Human liver cancer cell line HEP1 and the human embryonic kidney cell line HEK293a were obtained from American type culture collection (Manassas, VA, USA) and cultured according to their instructions. .. The expression construct for FUZ were purchased from Origene in a form of TrueORF® cDNA clones in pCMV6-Entry vector. cDNAs were shuttled into pLenti-C-mGFP (Origene) to create a lentivector encoding a fusion protein between the desired gene and mGFP. ..

    Article Title: Lung cancer methylation markers and uses thereof
    Article Snippet: The expression construct for a gene disclosed herein is purchased from Origene in a form of TrueORF® cDNA clones in pCMV6-Entry vector. cDNAs are shuttled into pLenti-C-mGFP (Origene) to create a lentivector encoding a fusion protein between the desired gene and mGFP. .. The expression construct for a gene disclosed herein is purchased from Origene in a form of TrueORF® cDNA clones in pCMV6-Entry vector. cDNAs are shuttled into pLenti-C-mGFP (Origene) to create a lentivector encoding a fusion protein between the desired gene and mGFP. ..

    Construct:

    Article Title: Liver cancer methylation markers and uses thereof
    Article Snippet: Cell Culture and Gene Transfections Human liver cancer cell line HEP1 and the human embryonic kidney cell line HEK293a were obtained from American type culture collection (Manassas, VA, USA) and cultured according to their instructions. .. The expression construct for FUZ were purchased from Origene in a form of TrueORF® cDNA clones in pCMV6-Entry vector. cDNAs were shuttled into pLenti-C-mGFP (Origene) to create a lentivector encoding a fusion protein between the desired gene and mGFP. ..

    Article Title: Lung cancer methylation markers and uses thereof
    Article Snippet: The expression construct for a gene disclosed herein is purchased from Origene in a form of TrueORF® cDNA clones in pCMV6-Entry vector. cDNAs are shuttled into pLenti-C-mGFP (Origene) to create a lentivector encoding a fusion protein between the desired gene and mGFP. .. The expression construct for a gene disclosed herein is purchased from Origene in a form of TrueORF® cDNA clones in pCMV6-Entry vector. cDNAs are shuttled into pLenti-C-mGFP (Origene) to create a lentivector encoding a fusion protein between the desired gene and mGFP. ..

    Article Title: Tumor-intrinsic KLRC1 exerts tumor-suppressive functions in colorectal cancer.
    Article Snippet: .. 2.6 Plasmid and siRNA transfection To generate a KLRC1 overexpression construct, the open reading frame (ORF) sequence was cloned into the pCMV6-entry vector (OriGene Technologies). .. For KLRC1 knockdown, transfection involved administering 7.2 μL of targeted siRNA (20 μM) with Lipofectamine 3000 reagent (Thermo Fisher Scientific) following the manufacturer's protocol.

    Clone Assay:

    Article Title: Liver cancer methylation markers and uses thereof
    Article Snippet: Cell Culture and Gene Transfections Human liver cancer cell line HEP1 and the human embryonic kidney cell line HEK293a were obtained from American type culture collection (Manassas, VA, USA) and cultured according to their instructions. .. The expression construct for FUZ were purchased from Origene in a form of TrueORF® cDNA clones in pCMV6-Entry vector. cDNAs were shuttled into pLenti-C-mGFP (Origene) to create a lentivector encoding a fusion protein between the desired gene and mGFP. ..

    Article Title: A new combination of 5’- and 3’-untranslated regions increases the expression of mRNAs in vitro and in vivo
    Article Snippet: .. Supplementary Materials are available in the online version of the paper: https://vavilovj-icg.ru/download/pict-2025-29/appx24.pdf Fragments containing MCS and UTRs were synthesized by the Laboratory of Synthetic Biology at the Institute of Chemical Biology and Fundamental Medicine (ICBFM) SB RAS and cloned into the pCMV6-Entry vector (OriGene, USA; see Supplementary Fig. S1) by means of restriction sites Psp124BI and XmaI (SibEnzyme, Russia). ..

    Article Title: Lung cancer methylation markers and uses thereof
    Article Snippet: The expression construct for a gene disclosed herein is purchased from Origene in a form of TrueORF® cDNA clones in pCMV6-Entry vector. cDNAs are shuttled into pLenti-C-mGFP (Origene) to create a lentivector encoding a fusion protein between the desired gene and mGFP. .. The expression construct for a gene disclosed herein is purchased from Origene in a form of TrueORF® cDNA clones in pCMV6-Entry vector. cDNAs are shuttled into pLenti-C-mGFP (Origene) to create a lentivector encoding a fusion protein between the desired gene and mGFP. ..

    Article Title: GPR39-mediated molecular signaling by bile acids
    Article Snippet: .. Mouse GPR39 cDNA was cloned into the pCMV6-Entry vector (OriGene), with an in-frame fusion to mCherry via a P2A linker. ..

    Article Title: Tumor-intrinsic KLRC1 exerts tumor-suppressive functions in colorectal cancer.
    Article Snippet: .. 2.6 Plasmid and siRNA transfection To generate a KLRC1 overexpression construct, the open reading frame (ORF) sequence was cloned into the pCMV6-entry vector (OriGene Technologies). .. For KLRC1 knockdown, transfection involved administering 7.2 μL of targeted siRNA (20 μM) with Lipofectamine 3000 reagent (Thermo Fisher Scientific) following the manufacturer's protocol.

    Article Title: GPR39 mediated molecular signaling by bile acids.
    Article Snippet: .. Mouse GPR39 cDNA was cloned into the pCMV6-Entry vector (OriGene), with an in-frame fusion to mCherry via a P2A linker. ..

    Synthesized:

    Article Title: A new combination of 5’- and 3’-untranslated regions increases the expression of mRNAs in vitro and in vivo
    Article Snippet: .. Supplementary Materials are available in the online version of the paper: https://vavilovj-icg.ru/download/pict-2025-29/appx24.pdf Fragments containing MCS and UTRs were synthesized by the Laboratory of Synthetic Biology at the Institute of Chemical Biology and Fundamental Medicine (ICBFM) SB RAS and cloned into the pCMV6-Entry vector (OriGene, USA; see Supplementary Fig. S1) by means of restriction sites Psp124BI and XmaI (SibEnzyme, Russia). ..

    Over Expression:

    Article Title: Tumor-intrinsic KLRC1 exerts tumor-suppressive functions in colorectal cancer.
    Article Snippet: .. 2.6 Plasmid and siRNA transfection To generate a KLRC1 overexpression construct, the open reading frame (ORF) sequence was cloned into the pCMV6-entry vector (OriGene Technologies). .. For KLRC1 knockdown, transfection involved administering 7.2 μL of targeted siRNA (20 μM) with Lipofectamine 3000 reagent (Thermo Fisher Scientific) following the manufacturer's protocol.



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